Rat FABP3 (Fatty Acid Binding Protein 3, Muscle and Heart) ELISA Kit
The Rat FABP3 (Fatty Acid Binding Protein 3) Muscle and Heart ELISA Kit is a specialized assay kit designed for the accurate measurement of FABP3 levels in rat muscle and heart tissue. This kit offers high sensitivity and specificity, ensuring precise and reliable results for research applications in the fields of muscle biology and heart disease.FABP3 is a protein that plays a critical role in fatty acid metabolism and transport in muscle and heart tissues. Abnormal levels of FABP3 have been linked to various cardiovascular diseases and muscle disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
With its advanced technology and user-friendly protocol, the Rat FABP3 Muscle and Heart ELISA Kit provides researchers with a powerful tool for investigating the roles of FABP3 in muscle and heart health, offering valuable insights into the mechanisms underlying these physiological processes.
Product Name:
Rat FABP3 (Fatty Acid Binding Protein 3, Muscle and Heart) ELISA Kit
SKU:
RTES00725
Target:
Rat FABP3 (Fatty Acid Binding Protein 3, Muscle and Heart)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat FABP3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat FABP3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat FABP3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat FABP3. You can calculate the concentration of Rat FABP3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-100
97-112
97-110
Average (%)
93
105
104
1:4
Range (%)
91-102
85-98
83-97
Average (%)
96
92
89
1:8
Range (%)
92-105
85-97
88-100
Average (%)
98
92
95
1:16
Range (%)
88-102
85-97
84-96
Average (%)
95
92
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-101
96
EDTA plasma (n=5)
95-109
101
Cell culture media (n=5)
85-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.49
1.35
3.83
0.48
1.35
4.05
Standard deviation
0.03
0.07
0.13
0.03
0.07
0.16
C V (%)
6.12
5.19
3.39
6.25
5.19
3.95
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat FABP3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat FABP3 in samples. No significant cross-reactivity or interference between Rat FABP3 and analogues was observed.
