Rat ESM1 (Endothelial Cell Specific Molecule 1) ELISA Kit
The Rat ESM1 (Endothelial Cell-Specific Molecule-1) ELISA Kit is specifically designed for the precise and reliable measurement of ESM1 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.ESM1 is a key protein involved in endothelial cell function and angiogenesis, playing a crucial role in promoting blood vessel formation and regulating cell behavior.
Dysregulation of ESM1 has been linked to various diseases, including cancer, cardiovascular disorders, and inflammatory conditions, making it a valuable biomarker for studying these diseases and developing potential therapeutic interventions.Overall, the Rat ESM1 ELISA Kit is a valuable tool for researchers working in the field of vascular biology and angiogenesis, providing reliable data to advance our understanding of ESM1-related mechanisms and potential treatment strategies.
Product Name:
Rat ESM1 (Endothelial Cell Specific Molecule 1) ELISA Kit
SKU:
RTES01101
Target:
Rat ESM1 (Endothelial Cell Specific Molecule 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ESM1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ESM1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ESM1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ESM1. You can calculate the concentration of Rat ESM1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-108
89-104
84-96
Average (%)
101
96
91
1:4
Range (%)
91-103
84-97
80-92
Average (%)
97
90
87
1:8
Range (%)
87-99
83-98
82-96
Average (%)
93
89
89
1:16
Range (%)
87-98
88-98
84-95
Average (%)
92
93
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
91
EDTA plasma (n=5)
88-103
94
Cell culture media (n=5)
87-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
263.47
569.17
1831.44
250.91
585.07
1930.93
Standard deviation
16.49
23.56
65.38
12.6
23.58
105.62
C V (%)
6.26
4.14
3.57
5.02
4.03
5.47
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat ESM1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ESM1 in samples. No significant cross-reactivity or interference between Rat ESM1 and analogues was observed.
