The Rat ES Endostatin CLIA Kit (RTES00198) is a cutting-edge diagnostic tool designed for the precise measurement of endostatin levels in rat samples. This kit offers excellent sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes. Endostatin is a key factor in regulating angiogenesis and inhibiting tumor growth. By measuring endostatin levels in rat serum, plasma, or tissue lysates, researchers can gain valuable insights into the development and progression of diseases such as cancer, cardiovascular disorders, and inflammatory conditions.
With its user-friendly protocol and reliable performance, the Rat ES Endostatin CLIA Kit (RTES00198) is a valuable asset for any laboratory studying angiogenesis and related physiological processes in rats. Order yours today and unlock new possibilities in your research endeavors.
Product Name:
Rat ES (Endostatin) CLIA Kit
SKU:
RTES00198
Target:
Rat ES (Endostatin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat ES. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ES and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ES, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat ES. You can calculate the concentration of Rat ES in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-115
95-108
91-106
Average (%)
105
101
98
1:4
Range (%)
103-115
100-117
101-116
Average (%)
109
109
109
1:8
Range (%)
99-111
100-114
85-96
Average (%)
104
106
90
1:16
Range (%)
92-103
93-103
91-103
Average (%)
98
98
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-113
103
EDTA plasma (n=5)
88-100
94
Cell culture media (n=5)
97-114
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
106.13
208.06
922.09
100.68
194.46
896.34
Standard deviation
13.61
23.51
68.33
10.73
17.77
75.65
C V (%)
12.82
11.3
7.41
10.66
9.14
8.44
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat ES concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ES in samples. No significant cross-reactivity or interference between Rat ES and analogues was observed.
