Rat ERR alpha (Estrogen Related Receptor Alpha) ELISA Kit
The Rat ERR Alpha (Estrogen-Related Receptor Alpha) ELISA Kit is specifically designed for the precise measurement of ERR Alpha levels in rat samples. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.ERR Alpha is a key transcription factor that plays a crucial role in energy metabolism, mitochondrial function, and cell differentiation. Dysregulation of ERR Alpha has been linked to metabolic disorders, cancer, and other diseases, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
Overall, the Rat ERR Alpha ELISA Kit provides researchers with a powerful tool for studying the role of ERR Alpha in various physiological and pathological processes, ultimately advancing our understanding of these complex biological mechanisms.
Product Name:
Rat ERR alpha (Estrogen Related Receptor Alpha) ELISA Kit
SKU:
RTES00840
Target:
Rat ERR alpha (Estrogen Related Receptor Alpha)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ERRα. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ERRα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ERRα, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ERRα. You can calculate the concentration of Rat ERRα in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-109
91-103
86-98
Average (%)
100
97
91
1:4
Range (%)
92-104
82-94
87-101
Average (%)
97
88
93
1:8
Range (%)
92-108
81-93
87-100
Average (%)
99
86
92
1:16
Range (%)
88-102
81-93
82-94
Average (%)
94
87
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-105
96
EDTA plasma (n=5)
87-100
94
Cell culture media (n=5)
88-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.53
3.69
0.53
1.5
3.57
Standard deviation
0.03
0.06
0.16
0.03
0.07
0.16
C V (%)
6
3.92
4.34
5.66
4.67
4.48
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat ERRα concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ERRα in samples. No significant cross-reactivity or interference between Rat ERRα and analogues was observed.
