Rat Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit

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SKU:
RTDL00321
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P06494
Sensitivity:
0.221ng/mL
Range:
0.625-40ng/mL
ELISA Type:
Sandwich
Synonyms:
CD340,ErbB2,HER-2/neu,HER2,NEU,MLN 19,NGL,TKR1,p185,V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 2,Neuro/Glioblastoma Derived Oncogene Homolog
Reactivity:
Rat
Sample Type:
Serum, Plasma And Other Biological Fluids
Frequently bought together:

Description

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Rat Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit

The Rat Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit is specifically designed for the accurate measurement of EGFR2 levels in rat serum, plasma, and tissue lysates. This kit offers excellent sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.EGFR2 is a key protein that plays a critical role in cell growth, division, and differentiation. Abnormalities in EGFR2 expression have been linked to various diseases, including cancer, making it a valuable biomarker for studying disease progression and potential treatment strategies.

This ELISA kit provides researchers with a reliable tool to study EGFR2 levels in rat samples, allowing for a better understanding of its role in disease pathology and facilitating the development of targeted therapies. Trust in the Rat EGFR2 ELISA Kit for accurate and dependable results in your research endeavors.

Product Name:Rat Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit
Product Code:RTDL00321
Size:96 Assays
Target:EGFR2
Synonyms:CD340, ErbB2, HER-2/neu, HER2, NEU, MLN 19, NGL, TKR1, p185, V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/Glioblastoma Derived Oncogene Homolog
Detection Method:Sandwich
Range:0.625-40ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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