The Rat Endothelin-1 (EDN1) ELISA Kit is specifically designed for the accurate and precise quantification of Endothelin-1 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring trustworthy and consistent results for a variety of research purposes.Endothelin-1 is a key peptide involved in vascular regulation and plays a crucial role in various physiological and pathological processes such as cardiovascular diseases, hypertension, and renal disorders.
By accurately measuring Endothelin-1 levels using this ELISA kit, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially develop new therapeutic strategies.Overall, the Rat Endothelin-1 (EDN1) ELISA Kit is a valuable tool for researchers studying vascular biology and related diseases in rat models, providing them with reliable data to advance their research efforts.
Product Name:
Rat Endothelin 1 (EDN1) ELISA Kit
Product Code:
RTDL00316
Size:
96 Assays
Target:
EDN1
Synonyms:
ET1, PPET1, Preproendothelin-1, Big endothelin-1
Detection Method:
Sandwich
Range:
7.812-500pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
