As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Product Name:
Rat EGFR/ErbB1 Recombinant Protein (RPES5227)
Product Code:
RPES5227
Size:
50µg
Species:
Rat
Expressed Host:
HEK293 Cells
Synonyms:
EGFR
Accession:
E7CXR8
Sequence:
Met1-Ser646
Fusion tag:
C-His
Activity:
1. Measured by its ability to bind recombinant human EGF-Fc in a functional ELISA.2. Measured by its ability to bind recombinant mouse EGF-Fc in a functional ELISA.
Protein Construction:
A DNA sequence encoding the rat EGFR (E7CXR8) (Met1-Ser646) was expressed, fused with a polyhistidine tag at the C-terminus.
Purity:
> 98 % as determined by SDS-PAGE
Mol Mass:
70.7 kDa
AP Mol Mass:
93-110 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
