SLC11A2,DCT1,NRAMP2,Natural resistance-associated macrophage protein 2,Solute Carrier Family 11 Member 2,Proton-Coupled Divalent Metal Ion Transporters
Reactivity:
Rat
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
The Rat Divalent Metal Transporter 1 (DMT1) ELISA Kit is a highly accurate and reliable tool for detecting levels of DMT1 in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research purposes.DMT1 is a key transporter protein responsible for regulating the uptake of divalent metals such as iron and zinc in cells. Dysregulation of DMT1 has been linked to various diseases and disorders, including iron overload conditions, neurodegenerative diseases, and metabolic disorders.
Understanding the levels of DMT1 in biological samples can provide valuable insights into these conditions and potential therapeutic targets.Overall, the Rat DMT1 ELISA Kit is a valuable tool for researchers studying metal transport mechanisms, iron metabolism, and related diseases in rats, offering accurate and reproducible results for advanced research applications.
Product Name:
Rat Divalent Metal Transporter 1 (DMT1) ELISA Kit
Product Code:
RTDL00305
Size:
96 Assays
Target:
DMT1
Synonyms:
SLC11A2, DCT1, NRAMP2, Natural resistance-associated macrophage protein 2, Solute Carrier Family 11 Member 2,Proton-Coupled Divalent Metal Ion Transporters
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
