Rat CTXI (Cross Linked C-telopeptide of Type I Collagen) ELISA Kit
The Rat CTXI (Cross-linked C-Telopeptide of Type I Collagen) ELISA Kit is specially designed for the precise measurement of CTXI levels in rat serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, guaranteeing consistent and accurate results, which are essential for various research applications.CTXI is a key marker of bone resorption and turnover, providing valuable insights into bone metabolism and health.
Elevated CTXI levels are often observed in conditions such as osteoporosis, arthritis, and other bone-related disorders, making this kit an indispensable tool for studying these diseases and exploring potential therapeutic interventions.Don't miss out on the opportunity to delve deeper into bone health and pathology with the Rat CTXI ELISA Kit from Assay Genie. Order yours today and unlock new discoveries in the field of bone research.
Product Name:
Rat CTXI (Cross Linked C-telopeptide of Type I Collagen) ELISA Kit
SKU:
RTES01009
Target:
Rat CTXI (Cross Linked C-telopeptide of Type I Collagen)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CTXⅠ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CTXⅠ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CTXⅠ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CTXⅠ. You can calculate the concentration of Rat CTXⅠ in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-114
96-111
96-108
Average (%)
104
103
101
1:4
Range (%)
86-101
84-98
81-96
Average (%)
93
90
88
1:8
Range (%)
92-105
85-100
84-98
Average (%)
97
92
91
1:16
Range (%)
91-105
82-95
83-94
Average (%)
97
89
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-110
102
EDTA plasma (n=5)
92-109
99
Cell culture media (n=5)
92-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.52
1.41
4.71
0.52
1.4
4.87
Standard deviation
0.03
0.08
0.15
0.03
0.08
0.17
C V (%)
5.77
5.67
3.18
5.77
5.71
3.49
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat CTXⅠ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CTXⅠ in samples. No significant cross-reactivity or interference between Rat CTXⅠ and analogues was observed.
