The Rat CTSL (Cathepsin L) CLIA Kit is a powerful tool for measuring cathepsin L levels in rat samples with high precision and accuracy. This kit is specifically designed for detecting the activity of Cathepsin L in various biological samples, such as serum, plasma, and tissue lysates, making it a valuable tool for researchers studying the role of Cathepsin L in disease pathogenesis.Cathepsin L is a lysosomal cysteine protease that plays a critical role in various cellular processes, including protein degradation, antigen processing, and tissue remodeling. Abnormal Cathepsin L activity is linked to a wide range of diseases, including cancer, cardiovascular diseases, and autoimmune disorders.
Therefore, the Rat CTSL CLIA Kit is an indispensable tool for investigating the role of Cathepsin L in disease progression and identifying potential therapeutic targets.With its high sensitivity and specificity, the Rat CTSL CLIA Kit delivers reliable and reproducible results, making it suitable for a wide range of research applications. By accurately measuring Cathepsin L levels in rat samples, researchers can gain valuable insights into the molecular mechanisms underlying disease pathogenesis and potentially develop novel diagnostic and therapeutic strategies.
Product Name:
Rat CTSL (Cathepsin L) CLIA Kit
SKU:
RTES00103
Target:
Rat CTSL (Cathepsin L)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CTSL. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CTSL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CTSL, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CTSL. You can calculate the concentration of Rat CTSL in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-115
101-118
95-109
Average (%)
109
108
103
1:4
Range (%)
90-107
98-113
100-114
Average (%)
98
103
106
1:8
Range (%)
102-117
101-116
100-117
Average (%)
109
107
108
1:16
Range (%)
97-110
101-116
91-103
Average (%)
103
107
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
102-116
108
EDTA plasma (n=5)
88-103
94
Cell culture media (n=5)
99-115
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
378.31
974.94
2718.88
340.48
893.1
2887.5
Standard deviation
42.37
84.14
309.14
33.61
84.84
310.98
C V (%)
11.2
8.63
11.37
9.87
9.5
10.77
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CTSL concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CTSL in samples. No significant cross-reactivity or interference between Rat CTSL and analogues was observed.
