Collagen triple helix repeat-containing protein 1, also known as Protein NMTC1, and CTHRC1, is a secreted protein that is glycosylated and highly conserved from lower chordates to mammals. CTHRC1 expression was not detectable in normal arteries. However, it is transiently expressed in the arterial wall in response to injury where it may contribute to vascular remodeling by limiting collagen matrix deposition and promoting cell migration. A short collagen motif with 12 Gly-X-Y repeats appears to be responsible for trimerization of the CTHRC1 protein and this renders the molecule susceptible to cleavage by collagenase. CTHRC1 overexpression caused a dramatic reduction in collagen type I mRNA and protein levels. Currently available data indicate that Cthrc1 expression in vascular cells regulates transforming growth factor beta responsiveness, thereby impacting transforming growth factor beta target genes, including collagens. Additionally, CTHRC1 increases bone mass as a positive regulator of osteoblastic bone formation and offers an anabolic approach for the treatment of osteoporosis.
Product Name:
Rat CTHRC1 Recombinant Protein (RPES3236)
Product Code:
RPES3236
Size:
20µg
Species:
Rat
Expressed Host:
HEK293 Cells
Synonyms:
CTHRC1
Accession:
NP_001258229.1
Sequence:
Met1-Lys230
Fusion tag:
C-His
Endotoxin:
<1.0 EU per µg protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the rat Cthrc1 (NP_001258229.1) (Met1-Lys230) was expressed with a polyhistidine tag at the C-terminus.
Purity:
> 90 % as determined by SDS-PAGE
Mol Mass:
22.8 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
