Rat CTF1 Recombinant Protein (RPPB0142)
- SKU:
- RPPB0142
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Rat
- Target:
- CTF1
- Synonyms:
- Cardiotrophin-1
- CT-1
- Ctf1
- Source:
- Escherichia Coli
- Uniprot:
- Q63086
Description
| Product Name: | Rat CTF1 Recombinant Protein |
| Product Code: | RPPB0142 |
| Size: | 10µg |
| Species: | Rat |
| Target: | CTF1 |
| Synonyms: | Cardiotrophin-1, CT-1, Ctf1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4. |
| Solubility: | It is recommended to reconstitute the lyophilized CTF1 in sterile 4mM HCl not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Cardiotrophin-1 Rat although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CTF1 Rat should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE and HPLC analyses. |
| Amino Acid Sequence: | MSQREGSLED HQTDSSFSFL PHLEAKIRQT HNLARLLTKY ADQLLEEYVQ QQGEPFGLPG FSPPRLPLAG LSGPAPSHAG LPVSERLRQD AAALSALPAL LDAVRRRQAE LNPRAPRLLR SLEDAARQVR ALGAAVETVL AALGAAARGP VPEPVATSAL FTSNSAAGVF SAKVLGLHVC GLYGEWVSRT EGDLGQLVPG GVA |
| Biological Activity: | The ED50 as determined by the dose-dependent proliferation of TF-1 cells was found to be < 0.5ng/ml, corresponding to a specific activity of > 2.0×106 units/mg. |
Cardiotrophin 1 (CT-1) is a 201 amino acid member of the interleukin-6 superfamily. It was identified by its ability to induce hypertrophic response in cardiac myocytes. CT-1 mRNA levels were found both in cardiac myocytes and in cardiac nonmyocytes. CT 1 was also detected in abundance in normal adult human lung and was expressed in both fetal and adult airway smooth muscle cells. CT 1 activates gp130 dependent signaling and stimulates the Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway to transduce hypertrophic and cytoprotective signals in cardiac myocytes.CT 1 has also a neurotrophic function. CTF1 deficiency causes increased motoneuron cell death in spinal cord and brainstem nuclei of mice during a period between embryonic day 14 and the first postnatal week. Moreover, CT-1 is a hepatocyte survival factor that efficiently reduces hepatocellular damage in animal models of acute liver injury. Cardiotrophin 1 expression is augmented after hypoxic stimulation and it can protect cardiac cells when added either prior to simulated ischaemia or at the time of reoxygenation following simulated ischaemia. Cardiotrophin 1 can induce expression of the protective heat shock proteins (hsps) in cardiac cells.Cardiotrophin-1 increased ventricular expression of ANP, brain natriuretic peptide (BNP) and angiotensinogen mRNA.Cardiophin 1 levels were significantly elevated in patients with heart failure, patients with dilatative cardiomyopathy, moderate/severe mitral regurgitation, stable and unstable angina and after acute myocardial infarction.
Cardiotrophin-1 Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 203 amino acids and having a molecular mass of 21.4kDa.The CTF1 Rat is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Induces cardiac myocyte hypertrophy in vitro. Binds to and activates the ILST/gp130 receptor. |
| NCBI Summary: | Interleukin-6 family cytokine with hypertrophic and protective effects in cardiac cells; also a neurotrophic factor [RGD, Feb 2006] |
| UniProt Code: | Q63086 |
| NCBI GenInfo Identifier: | 8393212 |
| NCBI Gene ID: | 29201 |
| NCBI Accession: | NP_058825.1 |
| UniProt Related Accession: | Q63086 |
| Molecular Weight: | 21,439 Da |
| NCBI Full Name: | cardiotrophin-1 |
| NCBI Synonym Full Names: | cardiotrophin 1 |
| NCBI Official Symbol: | Ctf1 |
| NCBI Protein Information: | cardiotrophin-1 |
| UniProt Protein Name: | Cardiotrophin-1 |
| UniProt Gene Name: | Ctf1 |
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