The Rat Complement 1 Inhibitor (C1INH) ELISA Kit is designed for the precise measurement of C1INH levels in rat serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures accurate and consistent results, making it an invaluable tool for various research purposes.C1INH is a key regulator of the complement system, playing a crucial role in controlling inflammation and immune response. Dysregulation of C1INH has been linked to various inflammatory disorders and autoimmune diseases, highlighting its importance as a potential therapeutic target.
By accurately quantifying C1INH levels, researchers can gain valuable insights into the underlying mechanisms of these conditions and develop new treatment strategies.Overall, the Rat Complement 1 Inhibitor (C1INH) ELISA Kit is a reliable and efficient tool for studying the role of C1INH in immune function and inflammatory diseases, providing researchers with the means to advance our understanding of these complex biological processes.
Product Name:
Rat Complement 1 Inhibitor (C1INH) ELISA Kit
Product Code:
RTDL00157
Size:
96 Assays
Target:
C1INH
Synonyms:
SERPING1, C1IN, C1-INH, C1NH, HAE1, HAE2, Serpin Peptidase Inhibitor Clade G Member 1, Angioedema Hereditary, C1 Inhibitor, C1 Esterase Inhibitor
Detection Method:
Competition
Range:
7.812-500ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
