Ciliary neurotrophic factor(CNTF) is a member of the cytokine family. It is a polypeptide hormone that have functions in promoting neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. It's actions appear to be restricted to the nervous system. Ciliary neurotrophic factor(CNTF) has biological effects through the activation of a multi-subunit receptor complex, consisting of an extracelluar CNTF binding subunit(CNTFα) and two transmembrane signal transduction proteins: glycoprotein gp130 and LIF receptor. CNTF is considered as a potent survival factor of neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. CNTF is also a survival factor for neurons of the peripheral sensory sympathetic, and ciliary ganglia. It has been reported that CNTF could be an agent that has therapeutic potential and possibly induces differentiation of large multipolar ganglionic phenotype in a subset of progenitors.
Product Name:
Rat CNTFR/CNTFR-alpha Recombinant Protein (RPES1461)
Product Code:
RPES1461
Size:
50µg
Species:
Rat
Expressed Host:
Baculovirus-Insect Cells
Synonyms:
Cntfr
Accession:
Q08406-1
Sequence:
Met 1-Pro 346
Fusion tag:
C-His
Activity:
Measured by its binding ability in a functional ELISA. Immobilized Rat CTNFR at 10 µg/ml (100 ?l/well) can bind biotinylated human CNTF with a linear ranger of 1.28-160 ng/ml.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method
Protein Construction:
A DNA sequence encoding the rat CNTFR (Q08406-1) (Met 1-Pro 346) was fused with a C-terminal polyhistidine tag.
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
