Rat Clusterin Recombinant Protein (RPPB0058)
- SKU:
- RPPB0058
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Rat
- Target:
- Clusterin
- Synonyms:
- CLI
- AAG4
- KUB1
- SGP2
- Source:
- Escherichia Coli
- Uniprot:
- P05371
Description
| Product Name: | Rat Clusterin Recombinant Protein |
| Product Code: | RPPB0058 |
| Size: | 10µg |
| Species: | Rat |
| Target: | Clusterin |
| Synonyms: | CLI, AAG4, KUB1, SGP2, SGP-2, SP-40, TRPM2, MGC24903, Complement-associated protein SP-40,40, Complement cytolysis inhibitor, NA1/NA2, Apolipoprotein J, Apo-J, Testosterone-repressed prostate message 2, TRPM-2. |
| Source: | Escherichia Coli |
| Physical Appearance: | White lyophilized (freeze-dried) powder. |
| Formulation: | Filtered (0.4µm) and lyophilized from 0.5mg/ml in 0.02M Tris buffer and 0.05M NaCl, pH 7.5. |
| Solubility: | It is recommended to add deionized water to a working concentration of 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. |
| Stability: | Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. |
| Purity: | Greater than 90% as determined by SDS PAGE. |
| Amino Acid Sequence: | MASMTGGQQM GRDPNSSSPF YFWMNGDRID SLLESDRQQS QVLDAMQDSF TRASGIIDTL FQDRFFTHEPQDIHHFSPMG FPHKRPHLLY PKSRLVRSLM PLSHYGPLSF HNMFQPFFDM IHQAQQAMDV QLHSPALQFPDVDFLKEGED DRTVCKEIRH NSTGCLKMKG QCEKCQEILS VDCSTNNPAQ ANLRQELNDS LQVAERLTQQYNELLHSLQS KMLNTSSLLE QALEHHHHHH |
Clusterin also named Apolipoprotein J (APO-J) is a 75-80 kD disulfide-linked heterodimeric protein containing about 30% of N-linked carbohydrate rich in sialic acid but truncated forms targeted to the nucleus have also been identified.The precursor polypeptide chain is cleaved proteolytically to remove the 22-mer secretory signal peptide and subsequently between residues 227/228 to generate the a and b chains. These are assembled in anti-parallel to give a heterodimeric molecule in which the cysteine-rich centers are linked by five disulfide bridges and are flanked by two predicted coiled-coil a-helices and three predicted amphipathic a-helices.Across a broad range of species clusterin shows a high degree of sequence homology ranging from 70% to 80%. It is nearly ubiquitously expressed in most mammalian tissues and can be found in plasma, milk, urine, cerebrospinal fluid and semen.It is able to bind and form complexes with numerous partners such as immunoglobulins, lipids, heparin, bacteria, complement components, paraoxonase, beta amyloid, leptin and others. Clusterin has been ascribed a plethora of functions such as phagocyte recruitment, aggregation induction, complement attack prevention, apoptosis inhibition, membrane remodeling, lipid transport, hormone transport and/or scavenging, matrix metalloproteinase inhibition.A genuine function of clusterin has not been defined. One tempting hypothesis says that clusterin is an extracellular chaperone protecting cells from stress induced insults caused by degraded and misfolded protein precipitates.Clusterin is up- or down regulated on the mRNA or protein level in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others.
The Clusterin Rat His-Tagged Fusion Protein, produced in E.coli, is 26.5kDa protein containing 215 amino acid residues of the APO-J Rat and 25 additional amino acid residues: N-terminal fusion of T7-Tag (16AA) and C-terminal fusion of His-Tag (9AA). (Underlined).
| UniProt Protein Function: | Functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. When secreted, protects cells against apoptosis and against cytolysis by complement. Intracellular forms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Promotes apoptosis when in the nucleus. Inhibits apoptosis when associated with the mitochondrial membrane by interference with BAX-dependent release of cytochrome c into the cytoplasm. Plays a role in the regulation of cell proliferation. |
| NCBI Summary: | The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders.[provided by RefSeq, May 2011] |
| UniProt Code: | P05371 |
| NCBI GenInfo Identifier: | 46048420 |
| NCBI Gene ID: | 24854 |
| NCBI Accession: | NP_444180.2 |
| UniProt Related Accession: | P05371 |
| Molecular Weight: | 51,375 Da |
| NCBI Full Name: | clusterin |
| NCBI Synonym Full Names: | clusterin |
| NCBI Official Symbol: | Clu |
| NCBI Official Synonym Symbols: | CLI; DAG; APOJ; SGP2; SP40; SGP-2; SP-40; Trpm2; Trpmb; TRPM-2; TRPM2B; RATTRPM2B |
| NCBI Protein Information: | clusterin |
| UniProt Protein Name: | Clusterin |
| UniProt Synonym Protein Names: | Dimeric acid glycoprotein; DAG |
| Protein Family: | Clusterin |
| UniProt Gene Name: | Clu |
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