Rat Cluster Of Differentiation 40 Ligand (CD40L) ELISA Kit

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SKU:
RTDL00193
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9Z2V2
Sensitivity:
0.061ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CD154,TRAP,HIGM1,IGM,IMD3,TBAM,T-BAM,TNFSF5,Gp39,TNF Superfamily Member 5,Hyper-IgM Syndrome,TNF-Related Activation Protein,T-Cell B-Cell Activating Molecule
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids
Frequently bought together:

Description

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Rat Cluster Of Differentiation 40 Ligand (CD40L) ELISA Kit

The Rat Cluster of Differentiation 40 Ligand (CD40L) ELISA Kit from AssayGenie is specifically designed for the precise detection of CD40L levels in rat samples. This comprehensive kit offers high sensitivity and accuracy, ensuring dependable and consistent results for a variety of research purposes.CD40L is a critical protein involved in immune response regulation and inflammation. Its role in various diseases like autoimmune disorders, cancer, and cardiovascular diseases makes it an important biomarker for understanding disease mechanisms and developing novel treatments.

With the Rat CD40L ELISA Kit, researchers can confidently measure CD40L levels in serum, plasma, and other sample types, making it an indispensable tool for studying immune responses and disease progression in rats. Trust AssayGenie for high-quality research tools and reliable results.

Product Name:Rat Cluster Of Differentiation 40 Ligand (CD40L) ELISA Kit
Product Code:RTDL00193
Size:96 Assays
Target:CD40L
Synonyms:CD154, TRAP, HIGM1, IGM, IMD3, TBAM, T-BAM, TNFSF5, Gp39, TNF Superfamily Member 5, Hyper-IgM Syndrome, TNF-Related Activation Protein, T-Cell B-Cell Activating Molecule
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)91-10296
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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