The Rat CK-18 (KRT18) Cytokeratin 18 ELISA Kit is specifically designed for the accurate quantification of rat cytokeratin 18 levels in serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.Cytokeratin 18 is a key intermediate filament protein that is essential for maintaining the structural integrity of epithelial cells. It is involved in cell migration, proliferation, and apoptosis, and dysregulation of cytokeratin 18 expression has been implicated in various diseases, including liver injury, liver fibrosis, and certain types of cancer.
By accurately measuring cytokeratin 18 levels, researchers can gain valuable insights into the pathophysiology of these diseases and potentially identify new therapeutic targets. The Rat CK-18 (KRT18) Cytokeratin 18 ELISA Kit is a valuable tool for studying cytokeratin 18 biology in rat models and advancing our understanding of these complex disease processes.
Product Name:
Rat CK-18/KRT18 (Cytokeratin 18) ELISA Kit
SKU:
RTES00838
Target:
Rat CK-18/KRT18 (Cytokeratin 18)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CK-18/KRT18. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CK-18/KRT18 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CK-18/KRT18, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CK-18/KRT18. You can calculate the concentration of Rat CK-18/KRT18 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-114
95-110
91-103
Average (%)
106
102
97
1:4
Range (%)
91-103
81-94
87-101
Average (%)
97
88
94
1:8
Range (%)
87-101
81-95
86-98
Average (%)
93
88
92
1:16
Range (%)
91-107
82-93
85-96
Average (%)
99
88
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-101
96
EDTA plasma (n=5)
91-106
97
Cell culture media (n=5)
90-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.52
1.46
4.61
0.54
1.37
4.99
Standard deviation
0.03
0.07
0.22
0.03
0.07
0.27
C V (%)
5.77
4.79
4.77
5.56
5.11
5.41
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat CK-18/KRT18 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CK-18/KRT18 in samples. No significant cross-reactivity or interference between Rat CK-18/KRT18 and analogues was observed.
