Rat Chemokine C-X-C-Motif Ligand 16 (CXCL16) ELISA Kit
The Rat Chemokine C-X-C Motif Ligand 16 (CXCL16) ELISA Kit is a reliable and sensitive assay designed for the precise measurement of CXCL16 levels in rat serum, plasma, and cell culture supernatants. This ELISA kit provides accurate and reproducible results, making it ideal for a variety of research applications in immunology, inflammation, and cancer studies.CXCL16 is an important chemokine involved in immune responses and inflammation, playing a crucial role in recruiting immune cells to sites of infection or injury. It is also implicated in the development and progression of various diseases, including atherosclerosis, autoimmune disorders, and cancer.
By accurately measuring CXCL16 levels, researchers can gain valuable insights into the mechanisms underlying these conditions and explore potential therapeutic targets.Overall, the Rat CXCL16 ELISA Kit offers a powerful tool for studying the role of CXCL16 in health and disease, providing researchers with reliable data to advance their understanding of immune responses and inflammatory processes in rat models.
Product Name:
Rat Chemokine C-X-C-Motif Ligand 16 (CXCL16) ELISA Kit
Product Code:
RTDL00273
Size:
96 Assays
Target:
CXCL16
Synonyms:
SR-PSOX, CXCLG16, SRPSOX, Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein, Small-inducible cytokine B16
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
