Rat CETP (Cholest Erolester Transfer Protein) CLIA Kit
The Rat CETP (Cholesteryl Ester Transfer Protein) CLIA Kit is a powerful tool for researchers looking to accurately measure CETP levels in rat serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and reproducible results for a variety of research applications.CETP is a key player in lipid metabolism, facilitating the transfer of cholesteryl esters between lipoprotein particles.
Dysregulation of CETP has been linked to cardiovascular diseases and dyslipidemia, making it a valuable biomarker for studying these conditions and developing therapeutic interventions.Don't miss out on the opportunity to deepen your understanding of CETP biology with the Rat CETP CLIA Kit from Assay Genie. Order yours today and take your research to the next level.
Product Name:
Rat CETP (Cholest Erolester Transfer Protein) CLIA Kit
SKU:
RTES00115
Target:
Rat CETP (Cholest Erolester Transfer Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CETP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CETP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CETP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CETP. You can calculate the concentration of Rat CETP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-99
92-107
96-110
Average (%)
91
98
101
1:4
Range (%)
84-96
85-96
94-110
Average (%)
91
91
101
1:8
Range (%)
93-105
88-100
93-108
Average (%)
99
93
101
1:16
Range (%)
87-100
96-112
94-109
Average (%)
92
104
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
97
EDTA plasma (n=5)
95-110
102
Cell culture media (n=5)
98-111
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
108.67
240.24
802.42
114.71
263.33
813.57
Standard deviation
9.63
26.98
63.87
13.01
29.78
70.37
C V (%)
8.86
11.23
7.96
11.34
11.31
8.65
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CETP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CETP in samples. No significant cross-reactivity or interference between Rat CETP and analogues was observed.
