Fc?RIIB is a low affinity receptor that recognizes the Fc portion of IgG. The human CD32 group consists of Fc?RIIA, Fc?RIIB, and Fc?RIIC proteins that share 94-99% sequence identity in their extracellular domains but differ substantially in their transmembrane and cytoplasmic domains. Fc?RII protein is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. Fc?RIIB has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) and delivers an inhibitory signal upon ligand binding. Ligation of Fc?RIIB on B cells down-regulates antibody production and in some circumstances may promote apoptosis. Co-ligation of Fc?RIIB on dendritic cells inhibits maturation and blocks cell activation. Fc?RIIB may also be a target for monoclonal antibody therapy for malignancies. Fc?RIIB plays an important negative-regulating role through modulating the signals from activation receptors.
Product Name:
Rat CD32b/FCGR2B Recombinant Protein (RPES1480)
Product Code:
RPES1480
Size:
50µg
Species:
Rat
Expressed Host:
HEK293 Cells
Synonyms:
FCRII,Fcgr2
Accession:
Q63203-1
Sequence:
Met 1-Pro 212
Fusion tag:
C-His
Activity:
Measured by its binding ability in a functional ELISA.Immobilized human rat FCGR2B-His at 10 µg/ml (100 ?l/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 0.3-0.8 µg/ml.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method
Protein Construction:
A DNA sequence encoding the rat FCGR2B (Q63203-1) extracellular domain (Met 1-Pro 212) was expressed, fused with a polyhistidine tag at the C-terminus.
Purity:
> 97 % as determined by SDS-PAGE
Mol Mass:
21.9 kDa
AP Mol Mass:
35-40 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
