CD133, also known as PROM1 and Prominin 1, is a pentaspan transmembrane glycoprotein which belongs to the prominin family. It localizes to membrane protrusions and is often expressed on adult stem cells. CD133 is known to play a role in maintaining stem cell properties by suppressing differentiation. CD133 binds cholesterol in cholesterol-containing plasma membrane microdomains. It is proposed to play a role in apical plasma membrane organization of epithelial cells. CD133 is also involved in regulation of MAPK and Akt signaling pathways. Mutations in PROM1 gene have been shown to result in retinitis pigmentosa and Stargardt disease. PROM1 gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Expression of this gene is also associated with several types of cancer.
Product Name:
Rat CD133/PROM1/Prominin 1 Recombinant Protein (RPES4417)
Product Code:
RPES4417
Size:
20µg
Species:
Rat
Expressed Host:
HEK293 Cells
Synonyms:
PROM1
Accession:
F1MAN1
Sequence:
Asn171-Tyr424
Fusion tag:
N-His
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method
Protein Construction:
A DNA sequence encoding the rat PROM1 (NP_001103607.1) (Asn171-Tyr424) was expressed with a polyhistidine tag at the N-terminus.
Purity:
> 90 % as determined by SDS-PAGE
Mol Mass:
30.9 kDa
AP Mol Mass:
47-57 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
