The Rat Carbonic Anhydrase IX (CA9) ELISA Kit is a highly sensitive and specific assay designed for the precise measurement of CA9 levels in rat serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for various research applications.Carbonic anhydrase IX is a key enzyme involved in the regulation of pH in cells and plays a critical role in various physiological processes. Abnormal expression of CA9 has been linked to a variety of diseases, including cancer and other metabolic disorders.
Therefore, accurate detection of CA9 levels is essential for studying the role of this enzyme in disease development and progression.The Rat Carbonic Anhydrase IX (CA9) ELISA Kit is a valuable tool for researchers seeking to better understand the function of CA9 and its implications in various pathological conditions. With its high sensitivity and specificity, this kit provides a reliable means of quantifying CA9 levels and advancing research in the field of enzymology and disease biology.
Product Name:
Rat Carbonic Anhydrase IX (CA9) ELISA Kit
Product Code:
RTDL00163
Size:
96 Assays
Target:
CA9
Synonyms:
CA-IX, CAIX, MN, pMW1, P54/58N, Carbonic Dehydratase, Renal cell carcinoma-associated antigen G250, RCC-Associated Protein G250, Carbonate dehydratase IX
Detection Method:
Sandwich
Range:
31.25-2,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
