Rat Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2) ELISA Kit
The Rat Cadherin EGF LAG Seven-Pass G-Type Receptor 2 (CELSR2) ELISA Kit is specifically designed for the quantitative detection of CELSR2 levels in rat serum, plasma, and tissue homogenates. This kit offers superior sensitivity and precision, ensuring accurate and reliable results for a variety of research applications.CELSR2, a cell adhesion molecule, plays a crucial role in cell signaling, adhesion, and tissue development. Its dysregulation has been linked to various diseases, including cancer, neurological disorders, and cardiovascular diseases.
The measurement of CELSR2 levels can provide valuable insights into disease mechanisms and potential therapeutic targets.Overall, the Rat Cadherin EGF LAG Seven-Pass G-Type Receptor 2 (CELSR2) ELISA Kit is a valuable tool for researchers studying cell adhesion, tissue development, and related diseases in rat models.
Product Name:
Rat Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2) ELISA Kit
Product Code:
RTDL00199
Size:
96 Assays
Target:
CELSR2
Synonyms:
CDHF10 EGFL2 Flamingo 1 MEGF3 Cadherin family member 10 Epidermal growth factor-like protein 2 Multiple epidermal growth factor-like domains protein 3
Detection Method:
Sandwich
Range:
0.78 -50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
