Rat Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit

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SKU:
RTDL00129
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P56819
Sensitivity:
0.062ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
ASP2,Beta Secretase,Memapsin 2,Aspartyl protease 2,Beta-site amyloid precursor protein cleaving enzyme 1,Membrane-associated aspartic protease 2
Reactivity:
Rat
Sample Type:
Serum, Plasma, Cerebrospinal Fluid And Other Biological Fluids
Frequently bought together:

Description

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Rat Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit

The Rat Beta-Site APP Cleaving Enzyme 1 (BACE1) ELISA Kit is specifically designed for the accurate quantitative measurement of BACE1 levels in rat serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, ensuring precise and reliable results for a variety of research applications.BACE1 is a key enzyme involved in the processing of amyloid precursor protein (APP) and the generation of amyloid beta peptides, which are known to play a crucial role in the pathogenesis of Alzheimer's disease.

By measuring BACE1 levels, researchers can gain valuable insights into the mechanisms underlying Alzheimer's disease and potential therapeutic interventions.Overall, the Rat BACE1 ELISA Kit is a valuable tool for studying the role of BACE1 in neurodegenerative disorders and for developing novel treatment strategies.

Product Name:Rat Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit
Product Code:RTDL00129
Size:96 Assays
Target:bACE1
Synonyms:ASP2, Beta Secretase, Memapsin 2, Aspartyl protease 2, Beta-site amyloid precursor protein cleaving enzyme 1, Membrane-associated aspartic protease 2
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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