Rat BDNF (Brain Derived Neurotrophic Factor) ELISA Kit
The Rat BDNF (Brain-Derived Neurotrophic Factor) ELISA Kit is specifically designed for the quantitative detection of BDNF levels in rat samples, including serum, plasma, and tissue homogenates. This ELISA kit offers exceptional sensitivity and specificity, delivering accurate and reproducible results for various research applications.BDNF is a key neurotrophic factor that supports the growth, survival, and function of neurons in the brain. It is involved in numerous physiological processes, including neuroplasticity, learning, and memory.
Dysregulation of BDNF expression has been linked to various neurological disorders, such as Alzheimer's disease, depression, and schizophrenia, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Rat BDNF ELISA Kit provides researchers with a powerful tool to investigate the role of BDNF in neurobiology and uncover novel insights into neurological diseases.
Product Name:
Rat BDNF (Brain Derived Neurotrophic Factor) ELISA Kit
SKU:
RTES00974
Target:
Rat BDNF (Brain Derived Neurotrophic Factor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat BDNF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BDNF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BDNF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat BDNF. You can calculate the concentration of Rat BDNF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-101
97-112
96-110
Average (%)
92
104
104
1:4
Range (%)
98-113
83-94
90-103
Average (%)
106
89
97
1:8
Range (%)
93-109
82-94
98-113
Average (%)
101
88
105
1:16
Range (%)
98-112
88-99
94-107
Average (%)
104
93
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-108
99
EDTA plasma (n=5)
92-107
99
Cell culture media (n=5)
83-98
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
107.4
179.2
874.5
103.2
162.3
829.5
Standard deviation
7
8.4
35.0
6.6
8.0
40.6
C V (%)
6.52
4.69
4.0
6.4
4.93
4.89
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat BDNF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat BDNF in samples. No significant cross-reactivity or interference between Rat BDNF and analogues was observed.
