Rat ATPase, H+/K+ Exchanging Beta Polypeptide (ATP4b) ELISA Kit
The Rat ATPase H+/K+ Exchanging Beta Polypeptide (ATP4B) ELISA Kit is specifically designed for the quantitative detection of ATP4B levels in rat samples including serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, resulting in accurate and reproducible results for various research applications.ATP4B is a vital component of the gastric proton pump, responsible for the secretion of hydrochloric acid in the stomach. Dysregulation of ATP4B expression has been linked to gastric disorders such as acid reflux, ulcers, and gastritis.
By measuring ATP4B levels, researchers can gain valuable insights into gastric function and potential therapeutic targets for related conditions.Overall, the Rat ATPase H+/K+ Exchanging Beta Polypeptide (ATP4B) ELISA Kit provides a reliable tool for studying the role of ATP4B in gastric physiology and pathology, offering researchers the ability to explore new avenues for treatment and management of gastric disorders.
Product Name:
Rat ATPase, H+/K+ Exchanging Beta Polypeptide (ATP4b) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
