The Rat Aquaporin 2 (Collecting Duct AQP2) ELISA Kit is a highly reliable and sensitive assay designed for the accurate detection of AQP2 levels in rat serum, plasma, and cell culture supernatants. This kit provides precise and reproducible results, making it a valuable tool for various research applications.Aquaporin 2 is a key protein involved in water reabsorption in the kidneys, particularly in the collecting ducts. Dysregulation of AQP2 expression has been associated with various renal disorders, making it a critical biomarker for studying these conditions and developing potential therapeutic interventions.
Overall, the Rat Aquaporin 2 (Collecting Duct AQP2) ELISA Kit is an essential tool for researchers studying renal physiology and pathology in rat models. With its high sensitivity and specificity, researchers can trust the accuracy of their results for advancing their scientific discoveries.
Product Name:
Rat Aquaporin 2, Collecting Duct (AQP2) ELISA Kit
Product Code:
RTDL00105
Size:
96 Assays
Target:
AQP2
Synonyms:
AQP-CD, WCH-CD, ADH water channel, Aquaporin-CD, Collecting duct water channel protein, Water channel protein for renal collecting duct
Detection Method:
Sandwich
Range:
0.625-40ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
