The Rat AQP-3 (Aquaporin-3) CLIA Kit is specifically designed for the quantitative detection of AQP-3 levels in rat serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for various research applications.Aquaporin-3 is a key protein in the Aquaporin family, playing a critical role in regulating water transport in cells and maintaining water homeostasis in the body. Dysregulation of AQP-3 has been implicated in various diseases, including skin disorders, kidney diseases, and gastrointestinal disorders, making it a valuable biomarker for studying these conditions and developing potential therapies.
With its user-friendly protocols and fast assay time, the Rat AQP-3 CLIA Kit is a valuable tool for researchers looking to investigate the function and regulation of AQP-3 in different biological contexts.
Product Name:
Rat AQP-3 (Aquaporin 3) CLIA Kit
SKU:
RTES00043
Target:
Rat AQP-3 (Aquaporin 3)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat AQP-3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat AQP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat AQP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat AQP-3. You can calculate the concentration of Rat AQP-3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-111
96-109
86-96
Average (%)
103
104
91
1:4
Range (%)
99-115
97-113
98-112
Average (%)
107
104
104
1:8
Range (%)
102-118
95-107
93-109
Average (%)
109
101
101
1:16
Range (%)
84-98
90-103
89-101
Average (%)
90
97
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
92
EDTA plasma (n=5)
99-113
106
Cell culture media (n=5)
99-116
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
202.22
389.32
1690.03
219.82
356.59
1821.17
Standard deviation
25.82
31.81
147.03
26.36
29.53
154.07
C V (%)
12.77
8.17
8.7
11.99
8.28
8.46
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat AQP-3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat AQP-3 in samples. No significant cross-reactivity or interference between Rat AQP-3 and analogues was observed.
