The Rat Angiotensin I (ANGI) ELISA Kit is a highly sensitive and specific assay designed for the accurate quantification of angiotensin I levels in rat serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it suitable for a variety of research applications.Angiotensin I is a key component in the renin-angiotensin system, playing a crucial role in regulating blood pressure and fluid balance in the body.
Dysregulation of angiotensin I levels has been implicated in various cardiovascular diseases, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.With its user-friendly protocol and high-quality components, the Rat Angiotensin I (ANGI) ELISA Kit is an essential tool for researchers looking to investigate the role of angiotensin I in disease pathogenesis and explore novel treatment strategies.
Product Name:
Rat Angiotensin I (AngI) ELISA Kit
Product Code:
RTDL00071
Size:
96 Assays
Target:
AngI
Synonyms:
Ang-I, Angiotensin-1
Detection Method:
Competition
Range:
15.625-1000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
85-100
92
EDTA plasma (n=5)
85-103
94
Heparin plasma (n=5)
87-99
93
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
