Rat Angiogenic Factor With G Patch And FHA Domains 1 (AGGF1) ELISA Kit
The Rat Angiogenic Factor with G-Patch and FHA Domains 1 (AGGF1) ELISA Kit is a powerful tool for researchers looking to study angiogenesis pathways in rats. This kit is specifically designed to measure AGGF1 levels in rat serum, plasma, and cell culture supernatants with high sensitivity and specificity, ensuring accurate and reliable results.AGGF1 is known for its role in promoting blood vessel formation and cell proliferation, making it a key player in various physiological and pathological processes.
By accurately quantifying AGGF1 levels, researchers can gain valuable insights into the mechanisms underlying angiogenesis and its implications in diseases such as cancer, cardiovascular disorders, and neurodegenerative conditions.Overall, the Rat AGGF1 ELISA Kit provides researchers with a valuable tool for studying angiogenesis in rats, offering a comprehensive understanding of this critical biological process and its potential implications in disease pathology and therapeutic development.
Product Name:
Rat Angiogenic Factor With G Patch And FHA Domains 1 (AGGF1) ELISA Kit
Product Code:
RTDL00041
Size:
96 Assays
Target:
AGGF1
Synonyms:
GPATC7, GPATCH7, VG5Q, Angiogenic factor VG5Q, G patch domain-containing protein 7, Vasculogenesis gene on 5q protein
Detection Method:
Competition
Range:
0.468-30ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
