Rat Amyloid Beta Precursor Protein Binding Protein B3 (APBB3) ELISA Kit

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SKU:
RTDL00087
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8R1C9
Sensitivity:
0.062ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
APB-B3,SRA,FE65L2,FE65-L2,Fe65L2 isoform 484,Alzheimer's beta-amyloid interaction protein,Amyloid beta (A4) protein-binding, family B, member 3, isoform CRA_g
Reactivity:
Rat
Sample Type:
Tissue Homogenates And Other Biological Fluids
Frequently bought together:

Description

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Rat Amyloid Beta Precursor Protein Binding Protein B3 (APBB3) ELISA Kit

The Rat APBB3 (Amyloid Beta Precursor Protein Binding Protein B3) ELISA Kit is a powerful tool for the accurate measurement of APBB3 levels in rat samples, including serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring precise and reproducible results for a wide range of research applications.APBB3 plays a crucial role in binding to the amyloid beta precursor protein, potentially impacting the pathogenesis of Alzheimer's disease and other neurodegenerative disorders.

By accurately quantifying APBB3 levels, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially develop targeted therapeutic interventions.Overall, the Rat APBB3 ELISA kit is a valuable resource for researchers investigating the role of APBB3 in neurodegenerative disorders and seeking to advance our understanding of Alzheimer's disease and related conditions.

Product Name:Rat Amyloid Beta Precursor Protein Binding Protein B3 (APBB3) ELISA Kit
Product Code:RTDL00087
Size:96 Assays
Target:APBB3
Synonyms:APB-B3, SRA, FE65L2, FE65-L2, Fe65L2 isoform 484, Alzheimer's beta-amyloid interaction protein, Amyloid beta (A4) protein-binding, family B, member 3, isoform CRA_g
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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