Rat Aminolevulinate Delta Synthase 1 (ALAS1) ELISA Kit
The Rat Aminolevulinate Delta Synthase 1 (ALAS1) ELISA Kit is a highly reliable and sensitive assay designed for the accurate detection of ALAS1 levels in rat samples such as serum, plasma, and tissue homogenates. This kit provides precise and reproducible results, making it suitable for a wide range of research applications.ALAS1 is a key enzyme involved in heme biosynthesis and is essential for the production of hemoglobin. Dysregulation of ALAS1 has been linked to various disorders including porphyrias and certain types of anemia.
Therefore, accurate measurement of ALAS1 levels is vital for understanding these conditions and identifying potential therapeutic targets.With its high quality components and easy-to-follow protocol, the Rat ALAS1 ELISA Kit is a valuable tool for researchers studying heme biosynthesis, porphyrias, anemia, and other related fields. It offers a reliable method for quantifying ALAS1 levels in rat samples, aiding in the advancement of scientific knowledge and the development of new treatments.
Product Name:
Rat Aminolevulinate Delta Synthase 1 (ALAS1) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
