Rat ALPL (Alkaline Phosphatase, Liver/Bone/Kidney) ELISA Kit
The Rat ALPL (Alkaline Phosphatase, Liver/Bone/Kidney) ELISA Kit is a highly sensitive and specific assay designed to accurately measure the levels of ALPL in rat serum, plasma, and tissue samples. This kit provides reliable and reproducible results, making it a valuable tool for researchers studying the role of ALPL in various biological processes.ALPL is a key enzyme involved in bone mineralization, liver function, and kidney health.
Dysregulation of ALPL activity has been associated with diseases such as osteoporosis, liver disorders, and kidney dysfunction, making it a critical biomarker for understanding and potentially treating these conditions.With its ease of use and robust performance, the Rat ALPL ELISA Kit is an essential resource for investigators seeking to unravel the intricate functions of ALPL in mammalian physiology and pathology.
Product Name:
Rat ALPL (Alkaline Phosphatase, Liver/Bone/Kidney) ELISA Kit
SKU:
RTES00914
Target:
Rat ALPL (Alkaline Phosphatase, Liver/Bone/Kidney)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ALPL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ALPL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ALPL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ALPL. You can calculate the concentration of Rat ALPL in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-112
96-114
99-115
Average (%)
105
104
105
1:4
Range (%)
92-108
82-95
88-101
Average (%)
100
88
94
1:8
Range (%)
92-104
83-93
88-104
Average (%)
97
88
95
1:16
Range (%)
91-104
86-97
85-101
Average (%)
96
92
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-99
91
EDTA plasma (n=5)
84-99
90
Cell culture media (n=5)
84-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.84
3.73
15.87
1.71
3.48
16.52
Standard deviation
0.11
0.17
0.57
0.1
0.21
0.66
C V (%)
5.98
4.56
3.59
5.85
6.03
4.0
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat ALPL concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ALPL in samples. No significant cross-reactivity or interference between Rat ALPL and analogues was observed.
