The Rat Alpha-1-AT (Alpha-1 Antitrypsin) ELISA Kit is a reliable tool for accurately measuring levels of Alpha-1 Antitrypsin in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Alpha-1 Antitrypsin is a critical protein that plays a key role in protecting tissues from damage caused by enzymes released during inflammation. Dysregulation of Alpha-1 Antitrypsin has been linked to various diseases, including liver and lung disorders.
Therefore, monitoring Alpha-1 Antitrypsin levels can provide valuable insights into disease progression and potential therapeutic interventions.Overall, the Rat Alpha-1-AT ELISA Kit is an indispensable tool for researchers studying the role of Alpha-1 Antitrypsin in health and disease, offering a reliable and efficient method for quantifying its levels in biological samples.
Product Name:
Rat alpha1-AT (Alpha 1-Antitrypsin) ELISA Kit
SKU:
RTES01048
Target:
Rat alpha1-AT (Alpha 1-Antitrypsin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
3.75 ng/mL
Detection range:
6.25-400 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat α1-AT. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat α1-AT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat α1-AT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat α1-AT. You can calculate the concentration of Rat α1-AT in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-112
84-95
96-110
Average (%)
104
90
103
1:4
Range (%)
92-106
80-89
84-96
Average (%)
100
85
91
1:8
Range (%)
90-105
83-94
87-99
Average (%)
98
89
92
1:16
Range (%)
93-109
86-98
87-102
Average (%)
99
92
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
96
EDTA plasma (n=5)
87-101
93
Cell culture media (n=5)
90-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
20.03
54.11
182.56
21.29
53.86
180.07
Standard deviation
1.37
3.09
5.55
1.11
2.96
9.9
C V (%)
6.84
5.71
3.04
5.21
5.5
5.5
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat α1-AT concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat α1-AT in samples. No significant cross-reactivity or interference between Rat α1-AT and analogues was observed.
