Rat Alkaline Phosphatase, Intestinal (ALPI) ELISA Kit
The Rat Alkaline Phosphatase Intestinal (ALPI) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of ALPI levels in rat serum, plasma, and tissue homogenates. This kit provides reliable and reproducible results, making it an excellent tool for researchers working on various gastrointestinal disorders, liver diseases, and bone metabolism studies.ALPI is a key enzyme found predominantly in the intestinal mucosa, liver, and bone tissues, playing a crucial role in the hydrolysis of phosphate esters and influencing bone mineralization.
Altered ALPI levels have been linked to conditions such as inflammatory bowel disease, liver disorders, and metabolic bone disorders, making it a valuable biomarker for assessing these conditions and developing therapeutic interventions.Overall, the Rat Alkaline Phosphatase Intestinal (ALPI) ELISA Kit from Assay Genie is a reliable and high-quality tool for studying ALPI levels in rat samples, offering researchers the precision and accuracy needed for their research endeavors.
Product Name:
Rat Alkaline Phosphatase, Intestinal (ALPI) ELISA Kit
Product Code:
RTDL00063
Size:
96 Assays
Target:
ALPI
Synonyms:
CIP, IAP, Calf Intestinal Alkaline Phosphatase
Detection Method:
Sandwich
Range:
0.781-50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
