Rat Aldehyde Dehydrogenase 9 Family, Member A1 (ALDH9A1) ELISA Kit
The Rat ALDH9A1 ELISA Kit is a highly reliable and sensitive assay designed for the accurate detection of ALDH9A1 levels in rat samples, including serum, plasma, and tissue homogenates. This kit offers exceptional specificity, ensuring reproducible results that are ideal for a variety of research applications.ALDH9A1, a member of the aldehyde dehydrogenase 9 family, plays a crucial role in the detoxification of aldehydes and the metabolism of amino acids. Dysregulation of ALDH9A1 has been linked to various diseases, including cancer, neurological disorders, and metabolic conditions, making it a valuable biomarker for studying these conditions and exploring potential treatment options.
The Rat ALDH9A1 ELISA Kit provides researchers with a powerful tool for investigating the role of ALDH9A1 in disease pathogenesis and progression, offering reliable and precise measurements that can aid in the development of novel therapeutic strategies.
Product Name:
Rat Aldehyde Dehydrogenase 9 Family, Member A1 (ALDH9A1) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
