The Rat Alcohol Dehydrogenase 7 (ADH7) ELISA Kit is a powerful tool for the quantitative measurement of ADH7 levels in rat serum, plasma, and cell lysates. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.ADH7 is an important enzyme involved in the metabolism of alcohol, playing a key role in the breakdown of ethanol in the liver. Dysregulation of ADH7 activity has been linked to various liver diseases and alcohol-related disorders, making it a valuable biomarker for studying these conditions and developing new therapeutic strategies.
With easy-to-follow instructions and quick assay protocols, the Rat ADH7 ELISA Kit is a convenient and reliable solution for researchers looking to investigate the role of ADH7 in alcohol metabolism and liver function. Trust in this kit to deliver accurate and reproducible data for your scientific investigations.
Product Name:
Rat Alcohol Dehydrogenase 7 (ADH7) ELISA Kit
Product Code:
RTDL00028
Size:
96 Assays
Target:
ADH7
Synonyms:
Alcohol dehydrogenase class 4 mu/sigma chain, Alcohol dehydrogenase class IV mu/sigma chain, Gastric alcohol dehydrogenase
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
