The Rat Adrenergic Receptor Beta-3 (ADRB3) ELISA Kit is a highly reliable and sensitive assay designed for the accurate measurement of ADRB3 levels in rat serum, plasma, and tissue lysates. This kit provides precise and reproducible results, making it an essential tool for research studies focusing on the role of ADRB3 in various physiological processes.ADRB3 is a key regulator of metabolism and energy expenditure, making it a potential target for obesity and metabolic disorders. By accurately measuring ADRB3 levels, researchers can gain valuable insights into the mechanisms underlying these conditions and develop novel therapeutic strategies.
Whether investigating the role of ADRB3 in metabolic regulation or studying its implications in cardiovascular diseases and neurodegenerative disorders, the Rat ADRB3 ELISA Kit is a valuable tool for precise and reliable biomarker analysis. Upgrade your research capabilities with the advanced technology and accuracy of this ELISA kit from Assay Genie.
Product Name:
Rat Adrenergic Receptor Beta 3 (ADRb3) ELISA Kit
Product Code:
RTDL00037
Size:
96 Assays
Target:
ADRb3
Synonyms:
BETA3AR, B3AR, Beta-3 adrenoreceptor
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-99
91
EDTA plasma (n=5)
80-99
89
Heparin plasma (n=5)
82-99
90
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
