Rat Adrenergic Receptor Alpha 2C (ADRa2C) ELISA Kit
The Rat Adrenergic Receptor Alpha 2C (ADRA2C) ELISA kit is a reliable tool for the accurate measurement of ADRA2C levels in rat serum, plasma, and other biological samples. This kit offers high sensitivity and specificity to ensure precise and reproducible results, making it suitable for a wide range of research applications.ADRA2C is a key receptor involved in the regulation of the sympathetic nervous system, playing a crucial role in modulating cardiovascular function, stress response, and other physiological processes. Dysregulation of ADRA2C has been implicated in various cardiovascular diseases, psychiatric disorders, and other pathological conditions, making it a valuable target for therapeutic intervention and biomarker discovery.
Whether investigating the molecular mechanisms underlying ADRA2C signaling or exploring potential therapeutic strategies targeting this receptor, the Rat Adrenergic Receptor Alpha 2C ELISA kit provides a valuable tool for researchers in the field of cardiovascular biology, neuroscience, and drug development. By accurately measuring ADRA2C levels in biological samples, this kit enables researchers to advance our understanding of ADRA2C function and its implications in health and disease.
Product Name:
Rat Adrenergic Receptor Alpha 2C (ADRa2C) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
