Rat Activin-A Recombinant Protein (RPPB0006)
- SKU:
- RPPB0006
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Rat
- Target:
- Activin-A
- Synonyms:
- Inhba
- Inhibin beta A
- FSH releasing protein
- Source:
- Escherichia Coli
- Uniprot:
- P18331
Description
| Product Name: | Rat Activin-A Recombinant Protein |
| Product Code: | RPPB0006 |
| Size: | 10µg |
| Species: | Rat |
| Target: | Activin-A |
| Synonyms: | Inhba, Inhibin beta A, FSH releasing protein. |
| Source: | Escherichia Coli |
| Physical Appearance: | Lyophilized freeze dried powder. |
| Formulation: | Rat Activin-A was lyophilized from a concentrated 1mg/ml protein solution containing 0.02% TFA. |
| Solubility: | Rat INHBA protein should be reconstituted in distilled pyrogen free water to a concentration of 100ug /ml which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Activin-A although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Activin-A should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95% as obsereved by SDS-PAGE. |
| Amino Acid Sequence: | MGLECDGKVN ICCKKQFFVS FKDIGWNDWI IAPSGYHANY CEGECPSHIA GTSGSSLSFH STVINHYRMR GHSPFANLKS CCVPTKLRPM SMLYYDDGQN IIKKDIQNMI VEECGCS |
| Biological Activity: | Biological activity is assessed by the ability to induce cytoxicity of MPC-11 cells and was found to be 1-1.5 ng/ml corresponding to a specific activity of 666,667-1,000,000 Units/mg. |
Activins are homodimers or heterodimers of the different Beta subunit isoforms, part of the TGFBeta family. Mature Activin A has two 116 amino acids residues BetaA subunits (BetaA-BetaA). Activin displays an extensive variety of biological activities, including mesoderm induction, neural cell differentiation, bone remodelling, haematopoiesis, and reproductive physiology. Activins takes part in the production and regulation of hormones such as FSH, LH, GnRH and ACTH. Cells that are identified to express Activin A include fibroblasts, endothelial cells, hepatocytes, vascular smooth muscle cells, macrophages, keratinocytes, osteoclasts, bone marrow monocytes, prostatic epithelium, neurons, chondrocytes, osteoblasts, Leydig cells, Sertoli cells, and ovarian granulosa cells.
Active form Activin-A Rat Recombinant produced in e.coli is a homodimeric, non-glycosylated, polypeptide chain containing 2 x 117 amino acids and having a molecular weight of 26.2kDa.The Active form Activin-A is purified by standard chromatographic techniques.
| UniProt Protein Function: | INHBA: Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. Belongs to the TGF-beta family. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted Cellular Component: extracellular space; extracellular region Molecular Function:identical protein binding; growth factor activity; protein heterodimerization activity; peptide hormone binding; hormone activity; cytokine activity; transforming growth factor beta receptor binding; receptor binding Biological Process: positive regulation of transcription, DNA-dependent; positive regulation of cellular protein metabolic process; activin receptor signaling pathway; mesodermal cell differentiation; palate development; negative regulation of cell cycle; regulation of apoptosis; odontogenesis; negative regulation of cell proliferation; hair follicle development; ovarian follicle development; cell cycle arrest; hemoglobin biosynthetic process; response to drug; regulation of follicle-stimulating hormone secretion; positive regulation of erythrocyte differentiation; male gonad development; progesterone secretion; regulation of transcription from RNA polymerase II promoter; mesoderm formation; regulation of MAPKKK cascade; hemopoietic progenitor cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; cell development; growth; G1/S transition of mitotic cell cycle |
| NCBI Summary: | subunit which can homodimerize or heterodimerize with Inhbb to regulate FSH secretion [RGD, Feb 2006] |
| UniProt Code: | P18331 |
| NCBI GenInfo Identifier: | 124281 |
| NCBI Gene ID: | 29200 |
| NCBI Accession: | P18331.1 |
| UniProt Related Accession: | P18331 |
| Molecular Weight: | 47,406 Da |
| NCBI Full Name: | Inhibin beta A chain |
| NCBI Synonym Full Names: | inhibin beta-A |
| NCBI Official Symbol: | Inhba |
| NCBI Protein Information: | inhibin beta A chain; activin A; activin beta-A chain |
| UniProt Protein Name: | Inhibin beta A chain |
| UniProt Synonym Protein Names: | Activin beta-A chain |
| Protein Family: | Inhibin |
| UniProt Gene Name: | Inhba |
| UniProt Entry Name: | INHBA_RAT |
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