Rat ACT alpha2 (Actin Alpha 2, Smooth Muscle) ELISA Kit
The Rat ACT Alpha2 (Actin Alpha 2 Smooth Muscle) ELISA Kit is specifically designed for the accurate quantification of ACT alpha2 levels in rat serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures reliable and reproducible results, making it ideal for various research applications.ACT alpha2 is an important protein involved in smooth muscle contraction and plays a key role in muscle physiology and pathophysiology.
Abnormal levels of ACT alpha2 have been linked to conditions such as smooth muscle-related disorders, making this kit an essential tool for studying these conditions and potential therapeutic interventions.Overall, the Rat ACT Alpha2 (Actin Alpha 2 Smooth Muscle) ELISA Kit is a valuable resource for researchers seeking to analyze and understand the role of ACT alpha2 in smooth muscle function and related diseases.
Product Name:
Rat ACT alpha2 (Actin Alpha 2, Smooth Muscle) ELISA Kit
SKU:
RTES01053
Target:
Rat ACT alpha2 (Actin Alpha 2, Smooth Muscle)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ACTα2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ACTα2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ACTα2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ACTα2. You can calculate the concentration of Rat ACTα2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
96-113
97-111
Average (%)
92
103
104
1:4
Range (%)
88-101
87-100
83-93
Average (%)
93
92
89
1:8
Range (%)
88-101
82-97
88-100
Average (%)
95
89
93
1:16
Range (%)
91-105
84-96
88-104
Average (%)
98
90
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
95
EDTA plasma (n=5)
87-102
94
Cell culture media (n=5)
84-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.55
22.09
81.47
9.42
22.25
89.17
Standard deviation
0.52
1.09
4.47
0.64
1.04
4.53
C V (%)
5.45
4.93
5.49
6.79
4.67
5.08
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat ACTα2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ACTα2 in samples. No significant cross-reactivity or interference between Rat ACTα2 and analogues was observed.
