IREB1,IRP1,IREBP,Iron regulatory protein 1,Iron-responsive element-binding protein 1,Cytoplasmic aconitate hydratase,Citrate hydro-lyase,Ferritin repressor protein
The Rat Aconitase 1 (ACO1) ELISA Kit is a powerful tool for accurately measuring ACO1 levels in rat samples including serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring precise and dependable results for various research applications.ACO1 is a key enzyme involved in the citric acid cycle and is essential for energy production in cells. Dysregulation of ACO1 has been implicated in various diseases including metabolic disorders, neurodegenerative diseases, and cancer.
Thus, the measurement of ACO1 levels can provide valuable insights into disease mechanisms and potential therapeutic strategies.Overall, the Rat Aconitase 1 (ACO1) ELISA Kit is a valuable tool for researchers studying the role of ACO1 in health and disease, offering accurate and reliable results for a wide range of experimental settings.
Product Name:
Rat Aconitase 1 (ACO1) ELISA Kit
Product Code:
RTDL00010
Size:
96 Assays
Target:
ACO1
Synonyms:
IREB1, IRP1, IREBP, Iron regulatory protein 1, Iron-responsive element-binding protein 1, Cytoplasmic aconitate hydratase, Citrate hydro-lyase, Ferritin repressor protein
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
