Rat A Disintegrin And Metalloprotease 8 (ADAM8) ELISA Kit
The Rat ADAM8 (A Disintegrin and Metalloprotease 8) ELISA Kit is a highly accurate and reliable assay designed for the detection of ADAM8 levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, providing precise and consistent results for a variety of research applications.ADAM8 is an important enzyme involved in various biological processes, including cell adhesion, migration, and signaling. Its dysregulation has been implicated in diseases such as cancer, inflammation, and autoimmune disorders, highlighting its potential as a valuable biomarker for studying and developing therapies for these conditions.
With the Rat ADAM8 ELISA Kit, researchers can confidently measure ADAM8 levels in rat samples, enabling further insights into the role of this enzyme in disease pathogenesis and progression. This kit is a valuable tool for investigators seeking to advance their understanding of ADAM8 and its potential implications in various pathological conditions.
Product Name:
Rat A Disintegrin And Metalloprotease 8 (ADAM8) ELISA Kit
Product Code:
RTDL00019
Size:
96 Assays
Target:
ADAM8
Synonyms:
CD156-A, MS2, CD156a, Disintegrin and metalloproteinase domain-containing protein 8
Detection Method:
Competition
Range:
15.6-1,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
