The Rat 6-PFK (Phosphofructokinase) CLIA Kit is a highly reliable and sensitive assay designed for the accurate measurement of 6-phosphofructokinase levels in rat samples. This kit is suitable for use with serum, plasma, and cell culture supernatants, providing researchers with a valuable tool for studying metabolic processes and energy production.6-phosphofructokinase is a key enzyme in glycolysis, playing a crucial role in regulating glucose metabolism and ATP production. Dysregulation of this enzyme has been linked to various metabolic disorders and diseases, making it an important target for research and drug development.
With its high specificity and reproducibility, the Rat 6-PFK CLIA Kit is a valuable resource for scientists working in the fields of metabolism, biochemistry, and drug discovery. Its easy-to-use format and quick results make it an ideal choice for high-throughput screening and quantitative analysis of 6-phosphofructokinase activity in rat samples.
Product Name:
Rat 6-PFK (Phosphofructokinase) CLIA Kit
SKU:
RTES00613
Target:
Rat 6-PFK (Phosphofructokinase)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PFK-A. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PFK-A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PFK-A, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PFK-A. You can calculate the concentration of Rat PFK-A in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-111
84-96
93-109
Average (%)
102
90
100
1:4
Range (%)
83-95
91-106
97-113
Average (%)
90
97
105
1:8
Range (%)
98-113
87-98
86-102
Average (%)
105
92
93
1:16
Range (%)
96-109
96-110
91-102
Average (%)
103
104
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
95
EDTA plasma (n=5)
94-106
99
Cell culture media (n=5)
94-112
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.93
1.87
7.87
0.86
1.7
7.1
Standard deviation
0.11
0.16
0.58
0.11
0.19
0.62
C V (%)
11.83
8.56
7.37
12.79
11.18
8.73
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PFK-A concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PFK-A in samples. No significant cross-reactivity or interference between Rat PFK-A and analogues was observed.
