The Rat 5-LO (Arachidonate 5-Lipoxygenase) ELISA Kit is specifically designed for the precise measurement of 5-LO levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.5-LO is an important enzyme involved in the synthesis of leukotrienes, lipid mediators that play a key role in inflammatory and immune responses.
Dysregulation of 5-LO activity has been implicated in various diseases, including asthma, arthritis, and cardiovascular disorders, highlighting its importance as a potential therapeutic target and biomarker for disease progression.Overall, the Rat 5-LO ELISA Kit provides researchers with a valuable tool for studying the role of 5-LO in health and disease, offering insights into potential treatment strategies and advancing our understanding of inflammatory pathways.
Product Name:
Rat 5-LO (Arachidonate 5-Lipoxygenase) ELISA Kit
SKU:
RTES00833
Target:
Rat 5-LO (Arachidonate 5-Lipoxygenase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat 5-LO. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat 5-LO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat 5-LO, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat 5-LO. You can calculate the concentration of Rat 5-LO in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
83-98
93-109
84-99
Average (%)
90
100
91
1:4
Range (%)
88-104
84-95
82-96
Average (%)
95
90
88
1:8
Range (%)
94-105
84-95
86-99
Average (%)
99
89
92
1:16
Range (%)
90-102
79-91
86-97
Average (%)
96
86
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-105
100
EDTA plasma (n=5)
91-102
96
Cell culture media (n=5)
86-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.54
1.44
4.11
0.57
1.48
3.99
Standard deviation
0.03
0.07
0.2
0.04
0.07
0.18
C V (%)
5.56
4.86
4.87
7.02
4.73
4.51
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat 5-LO concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat 5-LO in samples. No significant cross-reactivity or interference between Rat 5-LO and analogues was observed.
