Rat 5-Hydroxytryptamine Receptor 2C (HTR2C) ELISA Kit
The Rat 5-Hydroxytryptamine Receptor 2C (HTR2C) ELISA Kit is specifically designed for the accurate quantification of 5-HT2C receptor levels in rat samples including serum, plasma, and cell culture supernatants. With a high level of sensitivity and specificity, this kit provides reliable and reproducible results, making it ideal for a variety of research applications.The 5-HT2C receptor is a key player in serotonin signaling, influencing a range of physiological processes including mood regulation, appetite control, and cognitive function.
Dysregulation of this receptor has been implicated in various neuropsychiatric disorders such as depression, anxiety, and schizophrenia, making it a critical target for therapeutic interventions.By utilizing the Rat 5-Hydroxytryptamine Receptor 2C (HTR2C) ELISA Kit, researchers can gain valuable insights into the role of this receptor in health and disease, paving the way for the development of novel treatment strategies.
Product Name:
Rat 5-Hydroxytryptamine Receptor 2C (HTR2C) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
