Rat 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) ELISA Kit
The Rat 11 Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11B1) ELISA Kit is a highly sensitive and specific assay designed for the accurate measurement of HSD11B1 levels in rat serum, plasma, and tissue samples. This kit provides reliable and reproducible results, making it ideal for various research applications in the field of endocrinology and metabolism.HSD11B1 is a key enzyme involved in the conversion of inactive cortisone to active cortisol, playing a crucial role in regulating glucocorticoid metabolism and cortisol levels.
Dysregulation of HSD11B1 has been implicated in various metabolic disorders, including obesity, diabetes, and hypertension, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.With its precision and reliability, the Rat HSD11B1 ELISA Kit offers researchers a powerful tool for investigating the role of HSD11B1 in health and disease, providing valuable insights into the complex mechanisms underlying metabolic disorders.
Product Name:
Rat 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) ELISA Kit
Product Code:
RTDL00500
Size:
96 Assays
Target:
HSD11b1
Synonyms:
11DH, 11-beta-HSD1, HDL, HSD11, HSD11B, HSD11L, SDR26C1, Corticosteroid 11-beta-dehydrogenase isozyme 1, Short Chain Dehydrogenase/Reductase Family 26C,Member 1
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-99
91
EDTA plasma (n=5)
80-100
90
Heparin plasma (n=5)
82-99
90
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
