Rabbit TNF a Recombinant Protein (RPPB0997)
- SKU:
- RPPB0997
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Rabbit
- Target:
- TNF a
- Synonyms:
- Tumor necrosis factor
- Cachectin
- TNF-alpha
- Tumor necrosis factor ligand superfamily member 2
- Source:
- Escherichia Coli
- Uniprot:
- P04924
Description
| Product Name: | Rabbit TNF a Recombinant Protein |
| Product Code: | RPPB0997 |
| Size: | 20µg |
| Species: | Rabbit |
| Target: | TNF a |
| Synonyms: | Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, TNF, TNFA, TNFSF2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | TNF-alpha Rabbit was lyophilized after extensive dialysis against 20mM PB, pH7.4, 300mM NaCl. |
| Solubility: | It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Tumor Necrosis Factor-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-a should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Ala-Ser-Arg |
| Biological Activity: | The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is less than 0.03ng/ml, corresponding to a Specific Activity of 30,000,000 IU/mg. |
Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages. TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF's primary role is in the regulation of immune cells.Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.
Tumor Necrosis Factor-a Rabbit Recombinant consists of three identical polypeptide chains of 158 amino acids combined to form a compact, bell-shaped homotrimer. TNF-alpha was produced in E.Coli is a non-glycosylated, polypeptide chain having a molecular mass of 17.4 kDa for the individual subunit. The TNF-alpha is purified by standard chromatographic techniques.
| UniProt Protein Function: | Function: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. |
| UniProt Code: | P04924 |
| NCBI GenInfo Identifier: | 126722916 |
| NCBI Gene ID: | 100009088 |
| NCBI Accession: | NP_001075732.1 |
| UniProt Related Accession: | P04924 |
| Molecular Weight: | 25,817 Da |
| NCBI Full Name: | tumor necrosis factor |
| NCBI Official Symbol: | TNF |
| NCBI Official Synonym Symbols: | TNFA; TNFSF2 |
| NCBI Protein Information: | tumor necrosis factor; TNF-a; TNF-alpha; cachectin; tumor necrosis factor alpha; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor ligand superfamily member 2 |
| UniProt Protein Name: | Tumor necrosis factor |
| UniProt Synonym Protein Names: | Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a |
| Protein Family: | Tumor necrosis factor |
| UniProt Gene Name: | TNF |
| UniProt Entry Name: | TNFA_RABIT |
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