The PTD Pentosidine ELISA Kit is a reliable and accurate tool designed for the quantitative detection of PTD pentosidine levels in a variety of biological samples, including serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for researchers in the field.PTD pentosidine is a key biomarker for studying advanced glycation end products (AGEs) in various health conditions such as diabetes, chronic kidney disease, and aging-related disorders.
By measuring PTD pentosidine levels, researchers can gain valuable insights into the role of AGEs in disease progression and potential therapeutic interventions.Whether investigating the pathophysiology of AGE-related disorders or exploring new treatment strategies, the PTD Pentosidine ELISA Kit provides researchers with a valuable tool for advancing their research in this important area of study.
Product Name:
PTD (Pentosidine) ELISA Kit (UNES00057)
SKU:
UNES00057
Target:
PTD (Pentosidine) (UNES00057)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with PTD. During the reaction, PTD in the sample or standard competes with a fixed amount of PTD on the solid phase supporter for sites on the Biotinylated Detection Ab specific to PTD. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PTD in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-109
90-103
99-114
Average (%)
103
96
104
1:4
Range (%)
99-115
87-99
86-99
Average (%)
105
93
91
1:8
Range (%)
97-115
93-108
90-103
Average (%)
105
99
96
1:16
Range (%)
94-109
93-105
91-105
Average (%)
100
98
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
100
EDTA plasma (n=5)
89-103
96
Cell culture media (n=5)
86-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.3
106.1
442.5
44.0
106.6
426.9
Standard deviation
2.6
5.1
23.9
2.3
4.7
21.8
C V (%)
5.62
4.81
5.4
5.23
4.41
5.11
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of PTD concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes PTD in samples. No significant cross-reactivity or interference between PTD and analogues was observed.
