Porcine Insulin Like Growth Factor Binding Protein 3 (IGFBP3) ELISA Kit
The Porcine Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) ELISA Kit is a cutting-edge tool for the precise measurement of IGFBP3 levels in porcine samples, including serum, plasma, and cell culture supernatants. This advanced kit boasts exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research applications.IGFBP3 is a key regulator of insulin-like growth factors, playing a crucial role in cell growth and survival. Abnormal levels of IGFBP3 have been implicated in various diseases, including cancer, diabetes, and growth disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
With its superior performance and versatility, the Porcine IGFBP3 ELISA Kit is an indispensable tool for researchers seeking to investigate the role of IGFBP3 in porcine physiology and disease pathogenesis. Trust in this innovative kit to deliver reliable data and valuable insights for your research endeavors.
Product Name:
Porcine Insulin Like Growth Factor Binding Protein 3 (IGFBP3) ELISA Kit
Product Code:
PRDL00079
Size:
96 Assays
Target:
IGFBP3
Synonyms:
BP53, IBP3, Growth Hormone-Dependent Binding Protein, Acid Stable Subunit Of The 140 K IGF Complex
Detection Method:
Sandwich
Range:
3.125-200ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
